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KMID : 0360419700060020007
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Korean Journal of Pharmacology
1970 Volume.6 No. 2 p.7 ~ p.10
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Effect of Ginseng Saponin on Superprecipitation of Actomyosin
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林定圭/Lim, Jung Kyu
朴贊雄/金明石/劉巡鏞/Park, Chan Woong/Kim, Myung Suk/You, Soon Yong
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Abstract
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Actomyosin system undoubtedly represents the basic mechanism of muscular contraction. When actomyosin get reacts with ATP and Mg under conditions where ATP is hydrolyzed, this translucent gel shrinks and becomes more dense and opaque-it “superprecipitates”.
In this experiment, superprecipitation of natural actomyosin, extracted from rabbit back muscle, was followed by measuring the increase in turbidity at 545 m?? in the presence and absence of ginseng saponin. And ATP ase activity was measured by determining inorganic phosphate that is liberated during superprecipitation of actomyosin gel.
The experimental conditions were; 60 mM KCI, 2 mM MgCl₂, 20 mM Tris-maleate buffer, ph. 7.0, ATP: 10^(-4)~10^(-3)M. Ginseng saponis: 10^(-6)~5×10^(-5) gm/ml of reaction media, Actomyosin: 0.4 mg. protein/ml of reaction media, Temp. 25℃.
It was found that
1. the rate of increase 4 in turbidity was greater in the presence of ginseng saponin than in the absence.
2. the absorbancy at the maximum superprecipitation was greater in higher concentrations of ginseng saponin than in the absence but it was reversed in lower concentrations of ginseng saponin.
3. following the maximum superprecipitation, the absorbancy was decreared more rapidly in the presence of saponin than in the absence.
4. ginseng saponin had no significant influence in the rate of ATP hydrolysis of actomyosin.
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